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anjen chenn  (Addgene inc)


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    Structured Review

    Addgene inc anjen chenn
    Anjen Chenn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anjen+chenn/pCAG-Cre+(Plasmid+%2326647)/pmc11612790-341-41-81
    Average 91 stars, based on 6 article reviews
    anjen chenn - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages
    Article Snippet: .. AAV-CMVc-Cas9 was a gift from Juan Belmonte (Addgene plasmid # 106431). pCAG-Cre-IRES2-GFP was a gift from Anjen Chenn (Addgene plasmid # 26646). pUCmini-iCAP-PHP.eB was a gift from Viviana Gradinaru (Addgene plasmid # 103005). pHelper plasmid was a gift from the Cancer Research United Kingdom viral core facility. .. We PCR amplified the appropriate sequences to assemble a plasmid containing AAV2 ITRs flanking a CMV-Capsid-T2A-GFPpA construct with NEBuilder HiFi DNA assembly.

    Plasmid Preparation:

    Article Title: Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages
    Article Snippet: .. AAV-CMVc-Cas9 was a gift from Juan Belmonte (Addgene plasmid # 106431). pCAG-Cre-IRES2-GFP was a gift from Anjen Chenn (Addgene plasmid # 26646). pUCmini-iCAP-PHP.eB was a gift from Viviana Gradinaru (Addgene plasmid # 103005). pHelper plasmid was a gift from the Cancer Research United Kingdom viral core facility. .. We PCR amplified the appropriate sequences to assemble a plasmid containing AAV2 ITRs flanking a CMV-Capsid-T2A-GFPpA construct with NEBuilder HiFi DNA assembly.

    Article Title: Regulation of Cerebral Cortex Folding by Controlling Neuronal Migration via FLRT Adhesion Molecules.
    Article Snippet: .. Embryoswere electroporated at E13.5 using a combination of pCAG-Cre and pCALNL-DsRed plasmids (Matsuda andCepko, 2007). pCALNL-DsRed was a gift from Connie Cepko (Addgene plasmid # 13769) and pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647). .. After 48 hr, embryonic brains were dissected in ice cold sterile filtered and aerated (95% O2/5% CO2) dissection medium (15.6g/l DMEM/F12 (Sigma); 1.2g/l NaHCO3; 2.9g/l glucose (Sigma); 1%(v/v) penicillin streptomycin (GIBCO)).

    Article Title: Agarose hydrogel-mediated electroporation method for retinal tissue cultured at the air-liquid interface
    Article Snippet: .. Expression construct pCMV-eGFP was generated by the Levine lab. pCMV-mCherry was a gift from Roger Tsien (ClonTech plasmid # 632524, https://www.takarabio.com/documents/Vector%20Documents/PT3975-5_060412.pdf ). pCMV-Cre was a gift from David Liu (Addgene plasmid #123133; http://n2t.net/addgene:123133 ; RRID:Addgene 123133). pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647; http://n2t.net/addgene:26647 ; RRID:Addgene 26647), which was generated by Anjen Chenn by insertion of the Cre expression motif and deletion of the eGFP expression motif into the backbone of pCAG-GFP, a gift from Connie Cepko (Addgene plasmid #11150; http://n2t.net/addgene:11150 ; RRID: Addgene 11150). pBS513 EF1alpha-cre was a gift from Brian Sauer (Addgene plasmid # 11918; http://n2t.net/addgene:11918 ; RRID:Addgene 11918). ..

    Article Title: Light-Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near-Infrared Light.
    Article Snippet: Dr. D. P. Morales, E. N. Morgan, M. McAdams, A.. B. Chron, Prof. N. O. Reich Department of Chemistry and Biochemistry University of California Santa Barbara, CA 93106, USA E-mail: reich@chem.ucsb.edu J. E. Shin, Prof. J.. A. Zasadzinski Department of Chemical Engineering and Materials Science University of Minnesota Minneapolis, MN 55455, USA The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201800543. .. DsRed expression with a secondary transfection containing a plasmid-containing Cre recombinase was fused to GFP using the manufacturer’s protocol for Lipofectamine 3000 (Figure S1, Supporting Information). pCAG-Cre-IRES2-GFP was a gift from Anjen Chenn (Addgene plasmid no. 26646). ..

    Expressing:

    Article Title: Agarose hydrogel-mediated electroporation method for retinal tissue cultured at the air-liquid interface
    Article Snippet: .. Expression construct pCMV-eGFP was generated by the Levine lab. pCMV-mCherry was a gift from Roger Tsien (ClonTech plasmid # 632524, https://www.takarabio.com/documents/Vector%20Documents/PT3975-5_060412.pdf ). pCMV-Cre was a gift from David Liu (Addgene plasmid #123133; http://n2t.net/addgene:123133 ; RRID:Addgene 123133). pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647; http://n2t.net/addgene:26647 ; RRID:Addgene 26647), which was generated by Anjen Chenn by insertion of the Cre expression motif and deletion of the eGFP expression motif into the backbone of pCAG-GFP, a gift from Connie Cepko (Addgene plasmid #11150; http://n2t.net/addgene:11150 ; RRID: Addgene 11150). pBS513 EF1alpha-cre was a gift from Brian Sauer (Addgene plasmid # 11918; http://n2t.net/addgene:11918 ; RRID:Addgene 11918). ..

    Article Title: Light-Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near-Infrared Light.
    Article Snippet: Dr. D. P. Morales, E. N. Morgan, M. McAdams, A.. B. Chron, Prof. N. O. Reich Department of Chemistry and Biochemistry University of California Santa Barbara, CA 93106, USA E-mail: reich@chem.ucsb.edu J. E. Shin, Prof. J.. A. Zasadzinski Department of Chemical Engineering and Materials Science University of Minnesota Minneapolis, MN 55455, USA The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201800543. .. DsRed expression with a secondary transfection containing a plasmid-containing Cre recombinase was fused to GFP using the manufacturer’s protocol for Lipofectamine 3000 (Figure S1, Supporting Information). pCAG-Cre-IRES2-GFP was a gift from Anjen Chenn (Addgene plasmid no. 26646). ..

    Construct:

    Article Title: Agarose hydrogel-mediated electroporation method for retinal tissue cultured at the air-liquid interface
    Article Snippet: .. Expression construct pCMV-eGFP was generated by the Levine lab. pCMV-mCherry was a gift from Roger Tsien (ClonTech plasmid # 632524, https://www.takarabio.com/documents/Vector%20Documents/PT3975-5_060412.pdf ). pCMV-Cre was a gift from David Liu (Addgene plasmid #123133; http://n2t.net/addgene:123133 ; RRID:Addgene 123133). pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647; http://n2t.net/addgene:26647 ; RRID:Addgene 26647), which was generated by Anjen Chenn by insertion of the Cre expression motif and deletion of the eGFP expression motif into the backbone of pCAG-GFP, a gift from Connie Cepko (Addgene plasmid #11150; http://n2t.net/addgene:11150 ; RRID: Addgene 11150). pBS513 EF1alpha-cre was a gift from Brian Sauer (Addgene plasmid # 11918; http://n2t.net/addgene:11918 ; RRID:Addgene 11918). ..

    Generated:

    Article Title: Agarose hydrogel-mediated electroporation method for retinal tissue cultured at the air-liquid interface
    Article Snippet: .. Expression construct pCMV-eGFP was generated by the Levine lab. pCMV-mCherry was a gift from Roger Tsien (ClonTech plasmid # 632524, https://www.takarabio.com/documents/Vector%20Documents/PT3975-5_060412.pdf ). pCMV-Cre was a gift from David Liu (Addgene plasmid #123133; http://n2t.net/addgene:123133 ; RRID:Addgene 123133). pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647; http://n2t.net/addgene:26647 ; RRID:Addgene 26647), which was generated by Anjen Chenn by insertion of the Cre expression motif and deletion of the eGFP expression motif into the backbone of pCAG-GFP, a gift from Connie Cepko (Addgene plasmid #11150; http://n2t.net/addgene:11150 ; RRID: Addgene 11150). pBS513 EF1alpha-cre was a gift from Brian Sauer (Addgene plasmid # 11918; http://n2t.net/addgene:11918 ; RRID:Addgene 11918). ..

    Transfection:

    Article Title: Light-Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near-Infrared Light.
    Article Snippet: Dr. D. P. Morales, E. N. Morgan, M. McAdams, A.. B. Chron, Prof. N. O. Reich Department of Chemistry and Biochemistry University of California Santa Barbara, CA 93106, USA E-mail: reich@chem.ucsb.edu J. E. Shin, Prof. J.. A. Zasadzinski Department of Chemical Engineering and Materials Science University of Minnesota Minneapolis, MN 55455, USA The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201800543. .. DsRed expression with a secondary transfection containing a plasmid-containing Cre recombinase was fused to GFP using the manufacturer’s protocol for Lipofectamine 3000 (Figure S1, Supporting Information). pCAG-Cre-IRES2-GFP was a gift from Anjen Chenn (Addgene plasmid no. 26646). ..



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